Home » Posts tagged 'adenine'

Tag Archives: adenine

Recent Article Published: Unique H-bonding of Adenine with oxidatively damaged base 8-oxoguanosine enables specific recognition and repair by DNA glycosylase MutY.

Recent Article Published by Sheila David’s Lab: Unique Hydrogen Bonding of Adenine with the Oxidatively Damaged Base 8-Oxoguanine Enables Specific Recognition and Repair by DNA Glycosylase MutY.

Majumdar, C.; Mckibbin, P.L.; Krajewski, A.E.; Manlove, A.H.; Lee, J.K.; David, S.S.
J. Am. Soc. 2020. 142, 48, 20340–20350.

      DNA repair protein MutY employs specific interactions to differentiate OG:A basepairs from canonical G:C and T:A basepairs. Prior work from our lab has focused on understanding the structural requirements of OG on lesion recognition and catalysis, and we have shown that MutY relies on the exocyclic 2-amino group of OG to identify and distinguish OG:A from other basepairs. Additionally, we’ve shown that OG binding induces conformational changes that influence A excision.

     This new work uses structure-activity relationships (SARs) to identify the structural features of A that influence OG:A recognition, verification, base excision, and overall cellular repair. We correlate observed in vitro MutY activity on A analogue substrates with their experimental and calculated acidities to provide mechanistic insight into the factors influencing MutY base excision efficiency. Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. These results can also applied toward the development of MUTY/MUTYH specific inhibitors that may find utility in cancer therapeutics.

Click on the link or graphical abstract to find out more!

https://pubs.acs.org/doi/abs/10.1021/jacs.0c06767#

 

RSS Science Daily News

  • Cold plunges actually change your cells March 28, 2025
    Cold water immersion for seven days significantly improves cellular resilience and autophagic function, helping cells manage stress better. This adaptation could enhance health and longevity, potentially preventing diseases and slowing aging at a cellular level.
  • Artificial neurons organize themselves March 28, 2025
    Novel artificial neurons learn independently and are more strongly modeled on their biological counterparts. A team of researchers has programmed these infomorphic neurons and constructed artificial neural networks from them. The special feature is that the individual artificial neurons learn in a self-organized way and draw the necessary information from their immediate environment in the […]
  • A genetic tree as a movie: Moving beyond the still portrait of ancestry March 27, 2025
    Researchers have created a way to see your family tree as a movie rather than a still portrait by tracing where your ancestors moved across the globe over time. The statistical method can also be used to model disease spread and studying how animals move through geographic regions.
  • Melting ice, more rain drive Southern Ocean cooling March 27, 2025
    Researchers found increased meltwater and rain explain 60% of a decades-long mismatch between predicted and observed temperatures in the ocean around Antarctica.
  • Physics of irregular objects on inclined planes probed March 27, 2025
    How gravity causes a perfectly spherical ball to roll down an inclined plane is part of elementary school physics canon. But the world is messier than a textbook. Scientists have sought to quantitatively describe the much more complex rolling physics of real-world objects. They have now combined theory, simulations, and experiments to understand what happens […]

Contact:

Dr. Sheila S. David
ssdavid@ucdavis.edu
(530)-752-4280

Department of Chemistry
One Shields Ave.
Davis, CA 95616