Home » Posts tagged '8-oxoguanosine'
Tag Archives: 8-oxoguanosine
Recent Article Published: Unique H-bonding of Adenine with oxidatively damaged base 8-oxoguanosine enables specific recognition and repair by DNA glycosylase MutY.
Recent Article Published by Sheila David’s Lab: Unique Hydrogen Bonding of Adenine with the Oxidatively Damaged Base 8-Oxoguanine Enables Specific Recognition and Repair by DNA Glycosylase MutY.
Majumdar, C.; Mckibbin, P.L.; Krajewski, A.E.; Manlove, A.H.; Lee, J.K.; David, S.S.
J. Am. Soc. 2020. 142, 48, 20340–20350.
DNA repair protein MutY employs specific interactions to differentiate OG:A basepairs from canonical G:C and T:A basepairs. Prior work from our lab has focused on understanding the structural requirements of OG on lesion recognition and catalysis, and we have shown that MutY relies on the exocyclic 2-amino group of OG to identify and distinguish OG:A from other basepairs. Additionally, we’ve shown that OG binding induces conformational changes that influence A excision.
This new work uses structure-activity relationships (SARs) to identify the structural features of A that influence OG:A recognition, verification, base excision, and overall cellular repair. We correlate observed in vitro MutY activity on A analogue substrates with their experimental and calculated acidities to provide mechanistic insight into the factors influencing MutY base excision efficiency. Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. These results can also applied toward the development of MUTY/MUTYH specific inhibitors that may find utility in cancer therapeutics.
Click on the link or graphical abstract to find out more!
https://pubs.acs.org/doi/abs/10.1021/jacs.0c06767#
Science Daily News
- Scientists’ top 10 bee-magnet blooms—turn any lawn into a pollinator paradise July 7, 2025Danish and Welsh botanists sifted through 400 studies, field-tested seed mixes, and uncovered a lineup of native and exotic blooms that both thrill human eyes and lure bees and hoverflies in droves, offering ready-made recipes for transforming lawns, parks, and patios into vibrant pollinator hotspots.
- Whispers in the womb: How cells “hear” to shape the human body July 7, 2025Scientists found that embryonic skin cells “whisper” through faint mechanical tugs, using the same force-sensing proteins that make our ears ultrasensitive. By syncing these micro-movements, the cells choreograph the embryo’s shape, a dance captured with AI-powered imaging and computer models. Blocking the cells’ ability to feel the whispers stalls development, hinting that life’s first instructions […]
- Breakthrough battery lets physicists reverse entanglement—and rewrite quantum law July 7, 2025Scientists have finally uncovered a quantum counterpart to Carnot’s famed second law, showing that entanglement—once thought stubbornly irreversible—can be shuffled back and forth without loss if you plug in a clever “entanglement battery.”
- How a lost gene gave the sea spider its bizarre, leggy body July 7, 2025Scientists have decoded the sea spider’s genome for the first time, revealing how its strangely shaped body—with organs in its legs and barely any abdomen—may be tied to a missing gene. The detailed DNA map shows this ancient creature evolved differently from its spider and scorpion cousins, lacking genome duplications seen in those species. With […]
- Feeling mental exhaustion? These two areas of the brain may control whether people give up or persevere July 7, 2025When you're mentally exhausted, your brain might be doing more behind the scenes than you think. In a new study using functional MRI, researchers uncovered two key brain regions that activate when people feel cognitively fatigued—regions that appear to weigh the cost of continuing mental effort versus giving up. Surprisingly, participants needed high financial incentives […]