Home » Posts tagged 'Modified Nucleosides'

Tag Archives: Modified Nucleosides

Recent Article Published: Unique H-bonding of Adenine with oxidatively damaged base 8-oxoguanosine enables specific recognition and repair by DNA glycosylase MutY.

Recent Article Published by Sheila David’s Lab: Unique Hydrogen Bonding of Adenine with the Oxidatively Damaged Base 8-Oxoguanine Enables Specific Recognition and Repair by DNA Glycosylase MutY.

Majumdar, C.; Mckibbin, P.L.; Krajewski, A.E.; Manlove, A.H.; Lee, J.K.; David, S.S.
J. Am. Soc. 2020. 142, 48, 20340–20350.

      DNA repair protein MutY employs specific interactions to differentiate OG:A basepairs from canonical G:C and T:A basepairs. Prior work from our lab has focused on understanding the structural requirements of OG on lesion recognition and catalysis, and we have shown that MutY relies on the exocyclic 2-amino group of OG to identify and distinguish OG:A from other basepairs. Additionally, we’ve shown that OG binding induces conformational changes that influence A excision.

     This new work uses structure-activity relationships (SARs) to identify the structural features of A that influence OG:A recognition, verification, base excision, and overall cellular repair. We correlate observed in vitro MutY activity on A analogue substrates with their experimental and calculated acidities to provide mechanistic insight into the factors influencing MutY base excision efficiency. Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. These results can also applied toward the development of MUTY/MUTYH specific inhibitors that may find utility in cancer therapeutics.

Click on the link or graphical abstract to find out more!

https://pubs.acs.org/doi/abs/10.1021/jacs.0c06767#

 

RSS Science Daily News

  • Citizen scientists spot rare exploding star in real-time July 1, 2025
    Citizen scientists using the Kilonova Seekers platform spotted a stellar flash 2,500 times brighter than before, allowing astronomers to identify the exploding cataclysmic variable GOTO0650 within hours. Swift community follow-up captured X-ray, UV, and amateur telescope data, revealing the star’s rare “period-bouncer” stage.
  • Scientists just mapped how the body rejects pig organs—and how to stop it July 1, 2025
    Scientists have achieved an unprecedented look into how the human immune system attacks a transplanted pig kidney, using spatial molecular imaging to map immune activity down to the cellular level. They discovered early signs of rejection within 10 days and pinpointed key immune players—like macrophages—driving the response. Even more exciting: when targeted therapies were applied, […]
  • Scientists just found a sugar switch that protects your brain from Alzheimer's June 30, 2025
    Scientists have uncovered a surprising sugar-related mechanism inside brain cells that could transform how we fight Alzheimer’s and other dementias. It turns out neurons don’t just store sugar for fuel—they reroute it to power antioxidant defenses, but only if an enzyme called GlyP is active. When this sugar-clearing system is blocked, toxic tau protein builds […]
  • Ancient DNA reveals leprosy hit the Americas long before colonization June 30, 2025
    Leprosy’s tale stretches from 5,000-year-old skeletons in Eurasia to a startling 4,000-year-old case in Chile, revealing that the rare strain Mycobacterium lepromatosis haunted the Americas millennia before Europeans arrived. Armed with cutting-edge ancient-DNA sleuthing, scientists have pieced together remarkably well-preserved genomes that challenge the idea of leprosy as purely a colonial import and hint that […]
  • JWST unlocks 10-billion-year mystery of how galaxies shape themselves June 30, 2025
    Using the James Webb Space Telescope, scientists spotted thin and thick disks in galaxies as far back as 10 billion years ago—something never seen before. These observations reveal that galaxies first formed thick, chaotic disks, and only later developed the calm, thin disks seen in modern spirals like the Milky Way.

Contact:

Dr. Sheila S. David
ssdavid@ucdavis.edu
(530)-752-4280

Department of Chemistry
One Shields Ave.
Davis, CA 95616