Home » Posts tagged 'Modified Nucleosides'
Tag Archives: Modified Nucleosides
Recent Article Published: Unique H-bonding of Adenine with oxidatively damaged base 8-oxoguanosine enables specific recognition and repair by DNA glycosylase MutY.
Recent Article Published by Sheila David’s Lab: Unique Hydrogen Bonding of Adenine with the Oxidatively Damaged Base 8-Oxoguanine Enables Specific Recognition and Repair by DNA Glycosylase MutY.
Majumdar, C.; Mckibbin, P.L.; Krajewski, A.E.; Manlove, A.H.; Lee, J.K.; David, S.S.
J. Am. Soc. 2020. 142, 48, 20340–20350.
DNA repair protein MutY employs specific interactions to differentiate OG:A basepairs from canonical G:C and T:A basepairs. Prior work from our lab has focused on understanding the structural requirements of OG on lesion recognition and catalysis, and we have shown that MutY relies on the exocyclic 2-amino group of OG to identify and distinguish OG:A from other basepairs. Additionally, we’ve shown that OG binding induces conformational changes that influence A excision.
This new work uses structure-activity relationships (SARs) to identify the structural features of A that influence OG:A recognition, verification, base excision, and overall cellular repair. We correlate observed in vitro MutY activity on A analogue substrates with their experimental and calculated acidities to provide mechanistic insight into the factors influencing MutY base excision efficiency. Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. These results can also applied toward the development of MUTY/MUTYH specific inhibitors that may find utility in cancer therapeutics.
Click on the link or graphical abstract to find out more!
https://pubs.acs.org/doi/abs/10.1021/jacs.0c06767#
Science Daily News
- 'Microlightning' in water droplets may have sparked life on Earth March 14, 2025A study shows that electrical charges in sprays of water can cause chemical reactions that form organic molecules from inorganic materials. The findings provide evidence that microlightning may have helped create the building blocks necessary for early life on the planet.
- TOI-1453: Sub-Neptune in system of two exoplanets March 14, 2025Astronomers have discovered two exoplanets around TOI-1453, a star about 250 light years away. These two exoplanets, a super-Earth and a sub-Neptune, are common in the galaxy, yet are absent from our system. This discovery paves the way for future atmospheric studies to better understand these types of planets.
- Weighing in on a Mars water debate March 13, 2025Water once existed in abundance of at the surface of Mars. How much of that water has been stored in the planet's crust is still unclear, according to a new analysis.
- Spinning, twisted light could power next-generation electronics March 13, 2025Researchers have advanced a decades-old challenge in the field of organic semiconductors, opening new possibilities for the future of electronics. The researchers have created an organic semiconductor that forces electrons to move in a spiral pattern, which could improve the efficiency of OLED displays in television and smartphone screens, or power next-generation computing technologies such […]
- Study highlights noninvasive hearing aid March 13, 2025A study highlights a new approach in addressing conductive hearing loss. A team of scientists has designed a new type of hearing aid that not only improves hearing but also offers a safe, non-invasive alternative to implantable devices and corrective surgeries.