Home » Posts tagged 'Modified Nucleosides'
Tag Archives: Modified Nucleosides
Recent Article Published: Unique H-bonding of Adenine with oxidatively damaged base 8-oxoguanosine enables specific recognition and repair by DNA glycosylase MutY.
Recent Article Published by Sheila David’s Lab: Unique Hydrogen Bonding of Adenine with the Oxidatively Damaged Base 8-Oxoguanine Enables Specific Recognition and Repair by DNA Glycosylase MutY.
Majumdar, C.; Mckibbin, P.L.; Krajewski, A.E.; Manlove, A.H.; Lee, J.K.; David, S.S.
J. Am. Soc. 2020. 142, 48, 20340–20350.
DNA repair protein MutY employs specific interactions to differentiate OG:A basepairs from canonical G:C and T:A basepairs. Prior work from our lab has focused on understanding the structural requirements of OG on lesion recognition and catalysis, and we have shown that MutY relies on the exocyclic 2-amino group of OG to identify and distinguish OG:A from other basepairs. Additionally, we’ve shown that OG binding induces conformational changes that influence A excision.
This new work uses structure-activity relationships (SARs) to identify the structural features of A that influence OG:A recognition, verification, base excision, and overall cellular repair. We correlate observed in vitro MutY activity on A analogue substrates with their experimental and calculated acidities to provide mechanistic insight into the factors influencing MutY base excision efficiency. Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. These results can also applied toward the development of MUTY/MUTYH specific inhibitors that may find utility in cancer therapeutics.
Click on the link or graphical abstract to find out more!
https://pubs.acs.org/doi/abs/10.1021/jacs.0c06767#
Science Daily News
- Human activities have an intense impact on Earth's deep subsurface fluid flow April 24, 2024Hydrologists predict human-induced underground fluid fluxes to rise with climate change mitigation strategies like carbon sequestration.
- Holographic displays offer a glimpse into an immersive future April 24, 2024Researchers have invented a new optical element that brings us one step closer to mixing the real and virtual worlds in an ordinary pair of eyeglasses using high-definition 3D holographic images.
- This salt battery harvests osmotic energy where the river meets the sea April 24, 2024Estuaries -- where freshwater rivers meet the salty sea -- are great locations for birdwatching and kayaking. In these areas, waters containing different salt concentrations mix and may be sources of sustainable, 'blue' osmotic energy. Researchers report creating a semipermeable membrane that harvests osmotic energy from salt gradients and converts it to electricity. The new […]
- Making diamonds at ambient pressure April 24, 2024Researchers have grown diamonds under conditions of 1 atmosphere pressure and at 1025 degrees Celsius using a liquid metal alloy composed of gallium, iron, nickel, and silicon, thus breaking the existing paradigm. The discovery of this new growth method opens many possibilities for further basic science studies and for scaling up the growth of diamonds […]
- Eruption of mega-magnetic star lights up nearby galaxy April 24, 2024While ESA's satellite INTEGRAL was observing the sky, it spotted a burst of gamma-rays -- high-energy photons -- coming from the nearby galaxy M82. Only a few hours later, ESA's XMM-Newton X-ray space telescope searched for an afterglow from the explosion but found none. An international team realized that the burst must have been an […]