Home » Posts tagged 'Modified Nucleosides'

Tag Archives: Modified Nucleosides

Recent Article Published: Unique H-bonding of Adenine with oxidatively damaged base 8-oxoguanosine enables specific recognition and repair by DNA glycosylase MutY.

Recent Article Published by Sheila David’s Lab: Unique Hydrogen Bonding of Adenine with the Oxidatively Damaged Base 8-Oxoguanine Enables Specific Recognition and Repair by DNA Glycosylase MutY.

Majumdar, C.; Mckibbin, P.L.; Krajewski, A.E.; Manlove, A.H.; Lee, J.K.; David, S.S.
J. Am. Soc. 2020. 142, 48, 20340–20350.

      DNA repair protein MutY employs specific interactions to differentiate OG:A basepairs from canonical G:C and T:A basepairs. Prior work from our lab has focused on understanding the structural requirements of OG on lesion recognition and catalysis, and we have shown that MutY relies on the exocyclic 2-amino group of OG to identify and distinguish OG:A from other basepairs. Additionally, we’ve shown that OG binding induces conformational changes that influence A excision.

     This new work uses structure-activity relationships (SARs) to identify the structural features of A that influence OG:A recognition, verification, base excision, and overall cellular repair. We correlate observed in vitro MutY activity on A analogue substrates with their experimental and calculated acidities to provide mechanistic insight into the factors influencing MutY base excision efficiency. Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. These results can also applied toward the development of MUTY/MUTYH specific inhibitors that may find utility in cancer therapeutics.

Click on the link or graphical abstract to find out more!

https://pubs.acs.org/doi/abs/10.1021/jacs.0c06767#

 

RSS Science Daily News

  • One shot, seven days: Long-acting levodopa gel tackles Parkinson’s tremors July 14, 2025
    Researchers in Australia have created a biodegradable gel that delivers Parkinson’s medications through a single weekly shot, replacing the need for multiple daily pills. Injected just under the skin, the gel steadily releases levodopa and carbidopa for seven days, helping keep tremors and stiffness in check while easing side effects linked to fluctuating doses.
  • Can zebrafish help humans regrow hearing cells? July 14, 2025
    Zebrafish can regenerate sensory hair cells that humans permanently lose, like those in the inner ear linked to hearing and balance. New research reveals two specific genes that control how different supporting cells in zebrafish divide and regenerate, offering clues to how mammals might someday tap into similar regenerative powers.
  • It looked like nothing—then scientists found a world 10x the size of Jupiter July 14, 2025
    Astronomers have uncovered a massive, hidden exoplanet nestled in the dusty disc of a young star—MP Mus—by combining cutting-edge data from the ALMA observatory and ESA’s Gaia mission. Initially thought to be planet-free, the star’s surrounding disc appeared deceptively empty. But new observations and a telltale stellar “wobble” pointed to a Jupiter-sized gas giant forming […]
  • This AI-powered lab runs itself—and discovers new materials 10x faster July 14, 2025
    A new leap in lab automation is shaking up how scientists discover materials. By switching from slow, traditional methods to real-time, dynamic chemical experiments, researchers have created a self-driving lab that collects 10 times more data, drastically accelerating progress. This new system not only saves time and resources but also paves the way for faster […]
  • This Algorithm Just Solved One of Physics’ Most Infamous Problems July 14, 2025
    Using an advanced Monte Carlo method, Caltech researchers found a way to tame the infinite complexity of Feynman diagrams and solve the long-standing polaron problem, unlocking deeper understanding of electron flow in tricky materials.

Contact:

Dr. Sheila S. David
ssdavid@ucdavis.edu
(530)-752-4280

Department of Chemistry
One Shields Ave.
Davis, CA 95616