Home » Uncategorized » New Manuscript Published: The Zinc Linchpin Motif in the DNA Repair Glycosylase MUTYH: Identifying the Zn2+ Ligands and Roles in Damage Recognition and Repair.

New Manuscript Published: The Zinc Linchpin Motif in the DNA Repair Glycosylase MUTYH: Identifying the Zn2+ Ligands and Roles in Damage Recognition and Repair.

New Manuscript Published: The Zinc Linchpin Motif in the DNA Repair Glycosylase MUTYH: Identifying the Zn2+ Ligands and Roles in Damage Recognition and Repair.

A recent publication from the David, Siegel and Lim (Academia Sinica, Taiwan) labs (Nuñez et al., JACS, 2018) provides insight into the coordination sphere and critical role of a Zn2+ metal binding site in the DNA repair glycosylase MUTYH. Genome database mining and sequence alignment of MUTYH orthologs, along with computational modeling, identified and supported Zn2+ ligation by four Cys residues. Three of the Cys residues lie in an interdomain connector region unique to mammalian MutY enzymes, while the 4th Cys is located in close proximity to the Fe-S cluster DNA binding domain. The functional consequences of reduced Zn2+ chelation on MUTYH-mediated DNA repair activity evaluated using a battery of in vitro and cell-based assays revealed the importance of Zn-coordination in recognition of the damaged DNA substrate. The critical nature of the “Zinc Linchpin Motif” suggests additional functions unique to higher organisms in damage signaling and crosstalk with other DNA repair pathways.

More information at https://pubs.acs.org/doi/10.1021/jacs.8b06923.

Source:

J. Am. Chem. Soc. 2018, 140, 13260-13271.

Keywords: #Muty #Mutyh #BER #DNA #DNARepair #ZincLinchpinMotif #Zn2+ #8OG #DavidLab #UCDavis

 

 

 

 

 

RSS Science Daily News

  • In seconds, AI builds proteins to battle cancer and antibiotic resistance July 11, 2025
    Artificial intelligence is now designing custom proteins in seconds—a process that once took years—paving the way for cures to diseases like cancer and antibiotic-resistant infections. Australian scientists have joined this biomedical frontier by creating bacteria-killing proteins with AI. Their new platform, built by a team of biologists and computer scientists, is part of a global […]
  • Brighter, bolder, hotter: Why female guppies can't resist orange July 11, 2025
    Male guppies that glow with more orange aren’t just fashion-forward — they’re also significantly more sexually active. A UBC study reveals that brighter coloration is linked to virility and is genetically tied to brain development, suggesting a deeper evolutionary function. Researchers found that these bold hues aren't just for attracting mates, but are rooted in […]
  • Lemurs age without inflammation—and it could change human health forever July 10, 2025
    What if humans didn’t have to suffer the slow-burning fire of chronic inflammation as we age? A surprising study on two types of lemurs found no evidence of "inflammaging," a phenomenon long assumed to be universal among primates. These findings suggest that age-related inflammation isn’t inevitable and that environmental factors could play a far bigger […]
  • Your Brain’s Hidden Defenses Against Alzheimer’s July 10, 2025
    Scientists at UCSF combined advanced brain-network modeling, genetics, and imaging to reveal how tau protein travels through neural highways and how certain genes either accelerate its toxic journey or shield brain regions from damage. Their extended Network Diffusion Model pinpoints four gene categories that govern vulnerability or resilience, reshaping our view of Alzheimer’s progression and […]
  • Lasers capture the invisible dance of wind and waves July 10, 2025
    A laser-equipped research platform has, for the first time, photographed airflow just millimeters above ocean waves, revealing two simultaneous wind–wave energy-transfer tricks—slow short waves steal power from the breeze, while long giants sculpt the air in reverse. These crisp observations promise to overhaul climate and weather models by clarifying how heat, momentum, and greenhouse gases […]

Contact:

Dr. Sheila S. David
ssdavid@ucdavis.edu
(530)-752-4280

Department of Chemistry
One Shields Ave.
Davis, CA 95616